Learning disability weakens seriously the learning capacity of organization, and also impedes its knowledge transformation. Based on the elements of learning disability (the disability of structure, management, cultural, mental and execution), this research analyses the negative effects on knowledge transformation by learning disability, constructs the structural model and proposes a series of related research hypotheses. Then, we use empirical study to analyze synthetically the 259 valid questionnaires which was answered by related businesses. The results shows: as a whole, the restriction on the 4 aspect of knowledge transformation by the 5 elements of learning disability is obvious. But the restriction on the socialization process by the disability of management, the explicit process by the disability of structure and execution and the combination process by the disability of cultural is not obvious. So it is obvious that overall learning disability constraints the process of transformation of knowledge.