Based on the conservation of resource theory, using data from 391 dyads of three-stage questionnaires of employees and their immediate supervisors, this study explored the inhibiting mechanism of perceived dirty work on employee’ taking charge behavior, and examined the mediating effect of psychological availability, as well as the moderating effect of perceived investment in employee development. The results showed that: perceived dirty work had a significantly negative effect on employee’ taking charge behavior; psychological availability had a partial mediating effect in the relationship between perceived dirty work and employee’ taking charge behavior; perceived investment in employee development not only positively moderated the relationship between psychological availability and employee’ taking charge behavior, but also positively moderated the mediating effect of perceived dirty work on employee’ taking charge behavior through psychological availability.