Through two studies based on questionnaires and one multi-case study, the research aims to investigate the characteristics of top female leaders’ gender identity in enterprises and their influencing mechanism on subordinates’ personal identification with the leaders. The research finds that the gender identity of the top female leader is more androgynous, and the factor of femininity has a more significant impact on subordinates’ personal identification than the factor of masculinity. This research also finds that top female leaders with androgyny or femininity promote their subordinates’ personal identification by building trust in their ability and benevolence, and top female leaders with masculinity promote their subordinates’ personal identification through building trust in their ability and integrity. This means that for top female leaders to be effective, in addition to fully demonstrating their abilities, they must also pay attention to the sensitive and important role played by feminine traits such as benevolence.