Through examining four top American biotechnology pharmaceutical enterprises, this paper concludes seven critical success factors for biotechnology pharmaceutical enterprises using multiple cases study methods, which are “comprehensive organizing capacity”, “focusing on specialized market”, “financing capacity”, “excellent entrepreneur”, “continuous product innovation”, “location advantage” and “academic background of the team”. Meanwhile, we analyze the characters and relations among each factor. At last, revelations for Chinese biotechnology pharmaceutical enterprises are put forward based on above study and the characteristics of china.