Cultural self-knowledge is the basic feature of corporate culture. Enhancing the idea of cultural self-knowledge is one of the prerequisite and important contents. The cultural self-knowledge in enterprise culture construction is dealt with cultural self-knowledge of leaders and employees. The cultural self-knowledge of leaders plays key role in enterprise culture construction, and the one of employees does indispensable part in the construction. The cultural self-knowledge in the enterprise culture construction should be devoted much attention to.