R&D personnel gradually become an indispensable force in Information Age. Using the method of multilevel longitudinal study, this research investigates the mediating role work-family conflict and moderating role of family boundary strength which may influence the relationship between team-based human resource practices and turnover intentions among staff in R&D team. The study found that team-based human resource practices influences turnover intentions among staff in R&D team through the mediating role of work-family conflict. Besides, the more ambiguous family boundary strength is, the stronger impact of team-based human resource has on work-family conflict among staff in R&D team. Furthermore, the interaction of family boundary strength and team-based human resource practices affects turnover intentions through work-family conflict. The findings not only enrich related research of human resource management, but also provide an useful enlightenment on how to promote the retention intentions among staff in R&D team from the perspective of work-family relationship.