Abstract:This research is based on resourcebased view and identifies heterogeneous resources of new ventures. And further, researchers analyze resources’ effect on performance from the perspective of entrepreneurial opportunity. To meet the research goal, data were collected from 102 new ventures in Shandong province. Results indicate that human resource and relationship resource have positive effects on entrepreneurial performance; valuable entrepreneurial opportunity plays a mediating role. Theoretically, this study not only identifies heterogeneous resources that can influence the performance of new ventures which enriches the resourcebased theory, but also refines the Timmons model and promotes entrepreneurship research; practically, it provides theoretical guidance for entrepreneurs to realize the positive fit between resource and opportunity and ultimately achieve entrepreneurship success.